Neutrophilic segmented, band and juvenile granulocyte


Neutrophilic segment

This is the most frequent form of WBCs in a normal peripheral blood film. The cytoplasmatic granules are fine, have no distinct color, the nucleus is segmented into 3-5 parts.
The neutrophilic granules are sometimes so fine, that they are not visible. Their color is dull like that of the sand.
Sometimes the granules are coarser than usual, like here. This is still normal. If the neutrophilic granules are really coarse and have dark blue or black color they are called toxic granulation. This occurs when the peripheral blood is "left-shifted" which is a result of inflammation, infection. Toxic granulation is frequently accompanied by vacuolization of the cytoplasm.

Neutrophilic band

Neutrophilic band in normal peripheral blood
It is called band neutrophil because of its nonsegmented, long and narrow nucleus. The band-like nucleus can take any shape. The band neutrophil is called Stab in Hungary and in German medical texts. The word Stab means stick, which is somewhat misleading, because although the nucleus is long and narrow, but it is never straight.
Early band form. (It may also be considered a juvenile-band transition).
A band neutrophil with a nucleus that show the first signs of segmentation. (You may call this a band-segment transition.) There is no full agreement among different hematological schools about the difference between bands and segments. This explains why different hematological books report rather different percentage for bands in the WBC differential count. We call a granulocyte segmented form if there is at least one place along the nucleus where it is almost gets separated and is connected by a thin filament only. (The filament may be very short though.)
The nucleus of a band cell is long and narrow. The width of the nucleus can vary a bit, like here.


László L. Tornóci
Copyright © Inst. Pathophysiology, Semmelweis Medical University